CD66e (Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEA, DKFZp781M2392, Meconium antigen 100)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.

Human carcinoembryonic antigen

Human CD66e is a heavily glycosylated GPI anchored protein capable of heterophilic and homophilic adhesion and may play a role in metastasis of cancer cells. Soluble CD66e is also known as carcino embryonic antigen(CEA).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5x10e5 cultured UM-SCC cells (squamous cell carcinoma) per tube were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD66e antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.33 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.