CD40 (B Cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CDW40, MGC9013, p50, TNFRSF5, Tumor necrosis factor receptor superfamily member 5)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.

Murine L cells transfectants expressing human CD40

Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1). The CD40-CD154 interaction appears to have a role in artherosclerosis and cardiovascular disorders (3, 4, 5).

Applications:
Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5x10e5 cultured Raji cells per tube were incubated 45 min on ice with 80ul of anti-CD40 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse1.2 IgG1 negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.