CD66b (CEACAM-8, CEA-related cell adhesion molecule 8, CGM6, CEA gene family member 6, NCA-95, Nonspecific crossreacting antigen 95)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide.

CML leukocytes

CD66b is a 95-100kD GPI anchored molecule and is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for Immunohistochemistry (paraffin). Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:20-1:100. Use 10ul labels 5x10e5 cells or 100ul whole blood.
Immunohistochemistry (Frozen): 1:25-1:100. the epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Use acetone fixation for frozen sections.
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1903-13A: IgG, F(ab’)2, X-Adsorbed (PE) Pab: Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Recommended Positive Control Tissue:
Bone Marrow

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.