CD257 (B Lymphocyte Stimulator,BLyS,B Cell-activating Factor,BAFF,Dendritic Cell-derived TNF-like Molecule,DTL,THANK,TNF- and APOL-related Leukocyte Expressed Ligand 1,TALL1,TALL-1,TNFSF20,Tumor Necrosis Factor Ligand Su

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).

Recombinant correspnding to human CD257

Human CD257 (B cell activating factor, BAFF, TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation (2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells (3, 4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells (1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described (5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice (8, 9, 10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule (2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome (11).

Applications:
Suitable for use in FLISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
FLISA: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.