CDK2, phosphorylated (Thr14) (Cell Division Protein Kinase 2, p33 Protein Kinase)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr14 of human CDK2 (KLH).

CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.