Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, SEDC)

Antibodies / Antibodies-Collagen

-20°C

Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.

Human Collagen type II

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
Immunocytochemistry: Cultured human cells
ELISA: Native human collagen II
Dot Blot: Native human collagen II
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Human kidney, liver, skin, heart

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.