DDR1, NT (Epithelial Discoidin Domain-containing Receptor 1, Epithelial Discoidin Domain Receptor 1, Tyrosine Kinase DDR, Discoidin Receptor Tyrosine Kinase, Tyrosine-protein Kinase CAK, Cell Adhesion Kinase, TRK E, Protein-tyrosine Kinase 3A, P

Antibodies / Antibodies-Receptors

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 17-47 in the N-terminal region of human DDR1, UniProt Accession #NP_054699; Q03146. Species Sequence Homology: mouse

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin fixed, paraffin embedded tissues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.