NHERF1 (EBP50,ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD,Na(+)/H(+) exchange regulatory cofactor NHE-RF1,NHERF,NHERF1,NHERF-1,NPHLOP2,Regulatory cofactor of Na(+)/H(+) exchanger,Sodium-hydrogen exchanger r

Antibodies / Antibodies-Binding Proteins

4°C Do not freeze

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Recombinant protein corresponding to full length human EBP50.

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 5ug/ml
Western Blot: 2ug/ml under reducing conditions
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 cell line

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.