ERK1 (Extracellular Signal-regulated Kinase 1,ERK-1,ERT2,Insulin-stimulated MAP2 Kinase,MAP Kinase Isoform p44,Microtubule-associated Protein 2 Kinase,Mitogen-activated Protein Kinase 3,MAP Kinase 3,MAPK 3,MAPK3,Mitogen-

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Synthetic peptide corresponding to GGEPRRTEGVGP-C, from the N Terminus of human ERK1 (NP_002737.2; NP_001035145.1; NP_001103361.1).

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Application(s):
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA: 1:4000
Western Blot: 0.1-0.3ug/ml ~45kD band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kD according to NP_002737.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See E3452-46B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.