FRAP1 (S2481) (Serine/Threonine-protein Kinase mTOR,Mechanistic Target Of Rapamycin,FK506-binding Protein 12-rapamycin Complex-associated Protein 1,FKBP12-rapamycin Complex-associated Protein,Rapamycin Target Protein 1,R

Antibodies / Antibodies-Binding Proteins

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic non-phosphopeptide corresponding to amino acid residues surrounding S2481 of human FRAP1 (KLH).

FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.