ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS l

Antibodies / Antibodies-Ligands

4°C Do not freeze

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

Recombinant corresponding to human ICOS Ligand extracellular domain expressed in NS0 cells.

Human ICOS Ligand, also called B7-H2, B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse ICOS Ligand share ~49% amino acid identity. ICOS Ligand has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human ICOS Ligand is a 309 amino acid (aa) protein with a putative 18aa signal peptide, a 239aa extracellular domain, an 18aa transmembrane region and a 33aa cytoplasmic domain. Human ICOS Ligand is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e5 cells or 50ul of packed whole blood.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.