ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein Gl50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7 H2 ICOS L

Antibodies / Antibodies-Ligands

-20°C

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Recombinant protein corresponding to aa19-258 from human ICOS Ligand, expressed in NS0 cells (Accession #O75144

ICOS Ligand, also known as B7-related protein 1 (B7RP-1), is a costimulatory molecule that serves as a ligand for ICOS (Inducible Co-Stimulator).

Applications:
Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Neutralization: 1-4ug/ml yields one-half maximal inhibition (ND50) of the cell surface ICOS Ligand in the presence of 20ng/ml of anti-human CD3 and 3ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA activated human T cells as an assay.
Western Blot: 1ug/ml with recombinant human B7-H2 Fc Chimera
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.