IgG, H&L (HRP)

Pab

F(ab’)2

-20°C

Supplied as a lyophilized powder in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, protease/IgG free 0.1% kathon CG. Reconstitute with .55ml sterile dH2O. Labeled with horseradish peroxidase (HRP).

Purified human IgG H&L

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:200-1:5000
Western Blot: 1:200-1:5000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.