Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.

Nuclear protein preparation from the human cell line U937.50.

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10ug of A431 lysates.
Immunocytochemistry: 5-10ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilution determined by the researcher.

Recommended Secondary Antibodies:
I1904-02C: IgG, H&L (HRP) Pab Dk x Mo
I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk x Mo

Positive Control:
Tonsil tissue, A431 Cell Lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.