Ki-67 (Antigen Identified by Mab Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Antibodies / Antibodies-Proteins

4°C (-20°C Glycerol)

Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.

Synthetic peptide corresponding to an internal portion of the human protein (within aa1200-1300). [Swiss-Prot# P46013].

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:3200
Immunofluorescence: 1:50-1:200
Immunocytochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymph node

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.