Leucine-rich alpha-2-glycoprotein (LRG, HMFT1766, Leucine-rich alpha-2-glycoprotein 1, LRG1)

Antibodies / Antibodies-Carbohydrates, Glycoproteins

-20°C

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

Human Leucine-rich alpha-2-glycoprotein, covalently coupled to hemocyanin using glutaraldehyde

Leucine-rich alpha-2-glycoprotein (LRG) is a member of the leucine-rich repeat family. LRG is a plasma protein of approximately 50kD. LRG consists of 312aa. It has 1 galactosamine and 4 glucosamine oliogosacharides attached and has 2 intrachain disulfide bonds. Leucine comprises 66 of the 312aa, and LRG contains at least eight 24aa leucine-rich repeats. The consensus of these residues, termed leucine-rich repeats (LRR), has been identified as an LRR-containing domain. The primary function of LRRs is to provide versatile structural framework of the formation of protein-protein interactions. LRG is expressed during granulocyte differentiation. Although several family members of the leucine-rich repeat family have been shown to be involved in signal transduction, protein-protein interaction, or cell adhesion and development, a function for LRG remains elusive. LRG has been reported as a biomarker for certain diseases including microbial infections and cancer. LRG can be detected in human neutrophils and progenitor cells, but not in peripheral blood mononuclear cells.

Applications:
Suitable for use in ELISA and Immunopurification of human LRG. Other applications not tested.

Recommended Dilution:
ELISA: Antibody was used as detector for LRG on cytochrome C coated plates. Concentration antibody used was 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.