Lysosome Associated Membrane Protein 2 (LAMP 2, CD107b, Igp2, Igp110)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in BBS, pH 8.2. No preservatives or amine-containing buffer salts added.

Human Lysosome Associated Membrane Protein 2.

Lysosome Associated Membrane Protein 2 (LAMP 2), also known as CD107b, is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. LAMP 2 is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP 2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium (1-7).

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: ≤1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.