MAP Kinase 3 (Mitogen-activated Protein Kinase 3,MAPK 3,MAPK3,ERT2,Extracellular Signal-regulated Kinase 1,ERK1,ERK-1,Insulin-stimulated MAP2 Kinase,MAP Kinase Isoform p44,Microtubule-associated Protein 2 Kinase,Mitogen-

Mab

IgG2b, k

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Recombinant corresponding to aa1-137 human MAPK3 expressed in E. coli.

Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:250 - 500
Starting dilution: 1:500
Optimal dilutions to be determined by the researcher.

Hybridoma:
F0 myeloma cells with spleen cells from BALB/c mice

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.