Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

A fragment of the human nestin protein corresponding to residues 1464-1614.

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Westerm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Flow Cytometry: 1ug/1x10e6 cells
Immnohistochemistry(FFPE): 1:100-1:200
Immunofluorescence (IC):: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.