Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic peptide corresponding to 150aa fragment from human nestin conjugated to glutathione S-transferase.

Nestin is a class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.
Immunohistochemistry (frozen/paraffin): 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.
Western Blot: 1:1000-1:5000 using chemiluminescent detection.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Nestin transfected NS0 mouse myeloma cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.