Nicastrin

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.

A 19 aa peptide within the c-terminus of human nicastrin (1) was synthesized, coupled to KLH

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10, 000-50, 000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.