Nuclear Ribonucleoprotein

Antibodies / Antibodies-Nuclear Proteins

Blue Ice

Supernatant

30021010

snRNPs or small nuclear ribonucleoproteins, are particles that combine with pre-mRNA and various proteins to form spliceosomes (a type of large molecular complex). SnRNPs "recognize" the places along a strand of pre-mRNA and are essential in the removal of introns. These molecules are found within the cell's nucleus. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA. These molecules are usually about 150 nucleotides long. The snRNA is bound by a Ribonuclear protein (RNP) to activate its enzymatic activity. The precise beginnings and ends of introns on the primary transcripts are marked by signals so that they can be recognized and removed. The intron-boundary signals are recognized by a short bit of RNA found in curious RNA-protein complexes known as the small nuclear ribonucleoprotein particles - snRNPs, for short. At least four different kinds of snRNPs cooperate in most splicing. The RNA in these particles is like ribosomal RNA in that it is used directly, and has both an enzymatic and a structural role.

Applications:
Suitable for use in Immunohistochemistry and Indirect Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Immunofluorescence: 1:20-1:30, use 30-100ul per well/slide.
Immunohistochemistry (paraffin): 1:20-1:30, requires citrate acid microwave or HIER pretreatment.. Archival tissues will require more antibody than 24-48 fixed specimens.
Optimal dilutions to be determined by the researcher.

Controls:
Jurkat whole cell lysate, K-562 whole cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.