Parkin (RING-type E3 ubiquitin-protein ligase)

Antibodies / Antibodies-Ubiquitin Ligases

-20°C

Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.

A 23 amino acid peptide sequence mapping near the C-terminus of human parkin (1) was coupled to KLH

Parkinson's is the second most common neurodegenerative disease after Alzheimer's. Parkinson's is characterized by movement disorders-triad of rigidity, resting tremor and bradykinesia. It has been attributed to loss of neurons that produce neurotransmitter dopamine. Recently a new gene, Parkin, has been identified by positional cloning (1) that is responsible for a rare autosomal recessive form of Parkinsonism, AR-JP (2). Human Parkin gene encodes a protein of 465 AA (rat 465 aa; mouse 464 aa) with moderate similarity of ubiquitin at the N-terminus and RING-finger motif at the C-terminus. The gene has 12 exons, five of which (3-7) are deleted in AR-JP patients. Some other AR-JP patients have deletions affecting exon 4. Parkin is expressed in many tissues, including brain, heart, testis, and skeletal muscle. In the brain, parkin is expressed in various regions including substantia nigra. Parkin has recently been identified as an ubiquitin ligase (3).

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100, 000 using 50-100ng control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.