POLD2 (DNA Polymerase delta Subunit 2, DNA Polymerase delta Subunit p50)

Antibodies / Antibodies-Enzymes, Polymerase

-20°C

Supplied as a liquid in PBS, pH 7.2.

Full-length human POLD2 protein (1-469aa, NP_006221.1).

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multi-subunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2, POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).

Applications:
Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000; Detects a band at ~51.59kD using POLD2 transfected 293T lysate.
Immunofluorescence: 10ug/ml using HeLa cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.