RANKL (Receptor Activator of Nuclear Factor kappa B Ligand,Receptor Activator of NFkB Ligand,CD254,hRANKL2,hRANKL-2,Osteoclast Differentiation Factor,ODF,Osteoprotegerin Ligand,OPGL,sOdf,SOFA,TNF-related Activation-induc

Antibodies / Antibodies-RANK

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.

Recombinant human CD254.

CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.

Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. Studies have also revealed a link between CD254 and apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
ELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.