RANKL (Receptor Activator of Nuclear Factor kappa B Ligand,Receptor Activator of NFkB Ligand,CD254,hRANKL2,hRANKL-2,Osteoclast Differentiation Factor,ODF,Osteoprotegerin Ligand,OPGL,sOdf,SOFA,TNF-related Activation-induc

Antibodies / Antibodies-RANK

4°C Do Not Freeze

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Recombinant protein corresponding to aa64-245 from human RANKL expressed in NS0 cells (AAC51762).

TNF-related activation-induced cytokine is a 35kD (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317aa in length and contains a 47aa cytoplasmic region, a 21aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2, aa corresponding to 1-73 in isoform 1 are missing. In isoform 3, aa1-47 are missing. Human TRANCE ECD is 83% identical to mouse TRANCE ECD. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml using human primary differentiated CD4+ Th2 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.