RNP M1-M4 Nuclear Ribonucleoprotein, nRNP, hnRNP)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.

. This product is RNP autoantibody derived from donor human plasma.

Heterogeneous nuclear ribonucleoproteins (hnRNP) bind to nascent RNA polymerase II transcripts and are involved in transcript-specific packaging as well as alternative splicing of pre-mRNAs. hnRNPs also appear to influence mRNA metabolism and transport. In vivo, the M proteins bind to mRNA, and in vitro they bind avidly to poly(G) and poly(U) homopolymers. Four different isoforms of hnRNP are produced by alternative splicing: M1, M2, M3, and M4. Ongoing studies have theorized that hnRNPs may cause certain genetic diseases such as myotonic dystrophy, which is the most common form of adult muscular dystrophy. The identification of hnRNPs may therefore be important to determining the cause and cure for genetic diseases.

Applications:
Suitable for use in Double Immunodiffusion and ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Source:
Human plasma

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.