ROC1, CT (E3 Ubiquitin-protein Ligase RBX1, Protein ZYP, RING Finger Protein 75, RING-box Protein 1, Rbx1, Regulator of Cullins 1, RBX1, RNF75)

Antibodies / Antibodies-Cullin

-20°C

Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

Synthetic peptide corresponding to the C-terminus of human ROC1.

ROC1 and ROC2 are the two highly conserved RING finger proteins that are homologous to APC11, a subunit of the anaphase-promoting complex (APC). ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin1 to catalyze ubiquitin polymerization.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.