SSA1 (Tripartite Motif-containing 21,52kD Ribonucleoprotein Autoantigen Ro/SS-A,52kD Ro Protein,RING Finger Protein 81,RNF81,Ro(SS-A),RO52,Sjoegren Syndrome Type A Antigen,SSA,SS-A,TRIM21,Tripartite Motif-containing Prot

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.

Purified, E. coli-derived, recombinant human TRIM21 (aa1-475; Accession # P19474).

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52kD Ro Protein/Ro52, and RING finger protein 81) is a 52-56kD member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475aa in length and contains one E3 ligase RING finger domain (aa16-55), a B-Box type zinc-finger region (aa92-123), a coiled-coil region (aa128-238) and a C-terminal PRY/B30.2 Ig binding domain (aa268-465). TRIM21 is reported to form trimers. Over aa195-293, human TRIM21 exhibits 72aa identity with mouse TRIM21.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.