TLR2 (Toll Like Receptor 2, CD282, TIL4) (Biotin)

Antibodies / Antibodies-Toll Like Receptors (TLR)

-20°C

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.

Ba/F3 cells that stably expressed human Flag-tagged TLR2.

Toll-like receptors (TLR) are highly conserved throughout evolution and are implicated in the innate defense to many pathogens. Mammalian TLRs have been identified as type I transmembrane signaling receptors with pattern recognition capabilities. TLRs recognize pathogen-associated molecular patterns (PAMPs), expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression.

Among TLRs, TLR2 is an unique receptor recognizing lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as peptidoglycan, lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282). TLR2 is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Furthermore, TLR2 is detected in lung and fetal liver. In other tissues TLR2 levels are low.

Applications:
Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:50
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.