Vitronectin (VTN, Serum-spreading factor, S-protein, V75, VN, VNT)

Antibodies / Antibodies-Vitronectin

-20°C

Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride and 50% glycerol.

Human Vitronectin purified from plasma

Vitronectin (Vn), previously known as serum-spreading factor or S- protein, is a plasma and serum glycoprotein with a normal concentration ranging from 200 – 400ug/ml. It exists in both a 75kD single-chain form and a 65 + 10kD two-chain form. Vn can exist in a least two different conformational forms. The majority of Vn found in the circulation is present in the native (“closed”) form. In this form, most of the binding sites for other ligands are cryptic. The second form of Vn, the denatured (“open”, multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation. This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10kD light chain to the 65kD heavy chain of the two chain form. The liver is the primary site of Vn synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages. Vn plays an important role in a number of physiological and pathophysiological processes. It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis. Vn also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form.

Application:
Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.