CD95 (Apo-1 Antigen, APO1, Apoptosis-mediating Surface Antigen FAS, APT1, FAS, FASLG Receptor, Tumor Necrosis Factor Receptor Superfamily Member 6, TNF Receptor Superfamily Member 6, TNFRSF6)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile ddH20.

Synthetic peptide corresponding to aa87-109, YTDRKHYSDKCRRCAFCDEGHGL of rat CD95 (BSA).

FAS is a single-pass type I membrane protein and a member of the TNF-receptor superfamily. The interaction between this receptor and the FAS ligand (TNFSF6/FASLG) forms a death-inducing signalling complex that includes Fas-associated death domain protein (FADD), caspase 8 and caspase 10. Processing of the caspases in this complex leads to apoptosis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (Formalin fixed, paraffin, formalin/acetone fixed frozen): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.