Myeloperoxidase (MPO) (Biotin)

Mab

Blue Ice

Affinity Purified

30021010

MPO is a glycoprotein with an alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has more attracted clinical attention.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunofluorescence. Not recommended for Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:50 detects polymorphonuclear neutrophils. Paraffin sections are not recommended.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.