Tryptase (TPS, TPSAB1, Tryptase Alpha/Beta 1, Tryptase Alpha II, Tryptase Beta I, Tryptase-I, Tryptase-II, Tryptase-III)

Antibodies / Antibodies-Enzymes, Protease (Proteinase, Peptidase)

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol

Recombinant protein corresponding to Ser149-Phe273 from mouse TPS expressed in E. coli

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-5ug/ml
Immunohistochemistry (Frozen): 5-20ug/ml
Immunocytochemistry: 5-20ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control (Supplied):
142648A: Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol).
DAB: 10ul/well
ECL: 5ul/well
Store at -20°C. Stable for 6 months after receipt.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.