c-Fos (Fos)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a lyophilized powder in 0.05% sodium azide. Reconstitute with 200ul sterile PBS.

Synthetic peptide corresponding to aa4-17, SGFNADYEASSSRC of mouse c-Fos.

c-fos is a 55kD nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot (ECL): 10ug/ml. Detects a band at ~60kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.