CTHRC1 (Collagen Triple Helix Repeat Containing 1, Collagen Triple Helix Repeat-containing Protein 1) (Biotin)

Antibodies / Antibodies-Collagen

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.

Purified, NS0-derived, recombinant mouse CTHRC1 (aa33-245; Accession # Q3UAP6).

CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30kD, secreted glycoprotein that bears similarity to the C1q/TNFa-related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2, and to block TGFbeta-induced collagen type I and III synthesis. Mouse CTHRC1 is 245aa in length. It contains a 32aa signal sequence, a 16aa prosegment (aa33-48), and a 197aa mature region that shows one collagen-like domain (aa59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33kD, 245aa form, plus extracellular isoforms that are 28kD (aa33-245), 26kD (aa49-245), 20kD (aa98-245) and 18kD and 16kD in size, the last two representing variants of the 20kD form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa53-126. Full-length mouse CTHRC1 shares 99% and 93% aa identity with rat and human CTHRC1, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.