IgG, H&L (HRP)

Pab

F(ab’)2

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 500ul sterile ddH2O. Labeled with horseradish peroxidase (HRP)

Mouse IgG whole molecule.

Applications:
Suitable for use in Western and Dot Blot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:10, 000
ELISA: 1:10, 000-1:50, 000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.