Interleukin 33 Propeptide (IL-33, C9orf26, DVS27, IL1F11, IL-1F11, Interleukin-1 Family Member 11, Interleukin-33, NFEHEV, NFHEV, NF-HEV, Nuclear Factor from High Endothelial Venules)

Antibodies / Antibodies-Interleukin

-20°C

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Recombinant mouse IL-33 Propeptide aa1-108; Q8BVZ5 (E. coli).

IL-33, also known as NF-HEV and DVS 27, is an IL-1 family member that is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 alpha or beta in arterial smooth muscle, dermal fibroblasts and keratinocytes. IL-33 is synthesized as a 266aa, 32kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature mouse IL-33 shares 55% and 90% amino acid sequence identity with human and rat IL-33, respectively.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml on lysates of mouse peripheral lymph node tissue under reducing (R) and non-reducing (NR) conditions
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.