Neurofilament, Light Chain (NF-L)

Pab

IgY

-20°C

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide.

Three synthetic peptides corresponding to different regions of the NF-L gene product (KLH). Species sequence homology: human (P07196, NCBI) and mouse (P08551, NCBI)

Human neurofilament light chain (NF-L) is a 61.516 kD protein (543aa) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NF-L gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F).

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906IgY, Chicken Pab Rb x Ch
I1906-10IgY, Chicken (HRP) Rb x Ch
I1906-12IgY, Chicken (FITC) Rb x Ch
I1906-14IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.