TRIAD3A (E3 Ubiquitin Ligase TRIAD3A,E3 ubiquitin-protein ligase RNF216,RING finger protein 216,Ubiquitin-conjugating enzyme 7-interacting protein 1,UbcM4-interacting protein 83,Triad domain-containing protein 3,Rnf216,T

Antibodies / Antibodies-Transcription Factors, Zinc (Ring) Finger

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

A 15aa peptide located near mouse TRIAD3A amino-terminus.

TRIAD3A is a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.

Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.