Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

Antibodies / Antibodies-Obesity Proteins

4°C (-20°C Glycerol)

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

A 14aa peptide sequence near the C-terminus of mouse UCP2 (KLH coupled). Species Sequence Homology: rat, hamster: 92%; human, canine, porcine: 86%.

The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxoglutarate/malate and the citrate (tricarboxylate) carriers. UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold- and diet induced thermogenesis. Because of the restricted expression of UCP1 and the lack of BAT in adult animals and humans, UCP1 may not be important in body weight regulation/ energy expenditure in adults. Recently a novel UCP2, a homologue of UCP1, has been identified. In contrast with UCP1, UCP2 is widely expressed in adult human tissues and it is unregulated in WAT in response to fat intake. UCP2 is 59% homologous with UCP1. In the WAT of ob/ob and db/db mice, UCP2 expression is greatly elevated when compared to lean animals.

Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA:1:10, 000-1:100, 000 Use 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.

Control Peptide: U1621-06. Control peptide because of low MW (<3kD) is not suitable for Western. Suitable for ELISA or antibody blocking to confirm antibody specificity.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.