Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (AP)

Antibodies / Antibodies-Epitope Tag, Biotin

4°C Do Not Freeze

Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, 10mg/ml BSA, and 0.01% sodium azide, pH 8.0. Labeled with alkaline phosphatase.

Biotin (KLH).

Applications:
Suitable for use in ELISA, Western and Dot Blot, Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1000-1:5000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.