SARS E Protein (Severe Acute Respiratory Syndrome)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Synthetic peptide corresponding to amino acids at the amino-terminus of the SARS E protein (Genbank accession no. P59637)

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5).

Applications:
Can be used for the detection of SARS E protein in ELISA. It will detect 10ng of free peptide at 1mg/ml.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.