IgG, H&L (FITC)

Antibodies / Antibodies-IgG Conjugates

-20°C

Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).

Rabbit IgG

Applications:
Suitable for use in FLISA (Fluorescent), Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.

Absorption:
494nm

Emission:
518nm

FITC/Protein Ratio:
7.8 Moles FITC per Mole Antibody

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.