GFAP (Glial Fibrillary Acidic Protein)

Pab

IgY

-20°C

Supplied as a liquid, 5mM sodium azide.

Native GFAP purified from bovine spinal cord tissue

Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5000
Immunohistochemistry: 1:20, 000
Immunofluorescence (IC): 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906-10:IgY, Chicken (HRP) Rb x Ch I1906-12:IgY, Chicken (FITC) Rb x Ch I1906-14:IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.