Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.

Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160kD, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry: 1:10, 000
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.