CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Recombinant protein corresponding to Arg19-Lys560 of rat CD146 expressed in NS0 cells (Q9EPF2). Species Sequence Homology: human; 72%, mouse; 90%

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Applications:
Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells using PC-12 rat adrenal pheochromocytoma cell line.
Western Blot: 1ug/ml using recombinant rat MCAM/CD146 under non-reducing conditions only
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.