MAP2 (Microtubule Associated Protein 2, MAP-2)

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Three linear peptides corresponding to the N-terminal and central regions of rat MAP-2 (KLH).

The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP-2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurons. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml detects MAP-2 on 10ug of rat brain microsomal tissue lysate.
Immunohistochemistry (paraffin): 1:200 detects MAP-2 in rat brain tissue.
Immunocytochemistry (IC-IF): 1:1000 detected MAP-2 in E18 rat hippocampus neurons.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain microsomal tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.