Bcl 2 (B Cell Leukemia 2), Pro-Survival Family Antibody Sampler Kit, BioAssay™

Blue Ice

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml/ml BSA, 50% glycerol.

Synthetic peptides corresponding to residues surrounding phospho-Ser70, phospho-Ser56 and Gly41 of human Bcl-2, Asp61 of human Bcl-xL and Ser121 of human Mcl-1.

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4), which mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-surivival members including Bcl-2, Bcl-xL, Mcl-1, A1, and Bcl-w; pro-apoptotic proteins including Bax, Bak, and Bok; and “BH3 only” proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa, and Hrk. Interactions between death-promoting
and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the “BH3-only members” can bind to and antagonize the pro-survival proteins leading to
increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity and transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. Several phosphorylation sites have been identified within
Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (6).

Included antibodies:
B0807-02D1 Bcl 2, phosphorylated (Ser70) 20ulW, F H 28kDRabbit
B0807-02D2 Bcl-2, phosphorylated (Thr56) 20ul W H 28kDRabbit
B0807-02D3 Bcl-2 20ul W, IP, IHC H 28kDRabbit
B0807-02D4 Bcl-XL20ul W, IP, IHC H, M, R, Mk 30kDRabbit
B0807-02D5 Mcl-120ul W H 40kDRabbit
B0807-02D6 IgG (HRP)100ul Goat

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.