Cell Cycle Regulation BioAssay™ Sample Kit

Blue Ice

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Activity of the cyclin-dependent kinases is regulated by T-loop phosphorylation, abundance of their cyclin partners and association with cdk inhibitors of the Cip/Kip or INK family of proteins. p27 Kip1 derives its ability to enforce the G1 restriction point from its inhibitory binding to cdk2/cyclin E and other cdk/cyclin complexes. In growing cells, extracellular mitogenic stimuli induces the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRbdependent entry into S-phase. The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1S-phase gene expression. In association with cdk2 complexes, p21 serves to inhibit kinase activity and block progression through G1/S, but it may also enhance assembly and activity in complexes of cdk4 or cdk6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression.

Kit Components
1. p15 INK4B Pab Rb x 1x20ul
2. p16 INK4A Pab Rb x 1x20ul
3. CDK4 Mab Mo x 1x20ul
4. CDK6 Mab Mo x 1x20ul
5. p27 Kip1 Pab Rb x 1x20ul
6. Cyclin D1 Mab Mo x 1x20ul
7. Cyclin D3 Mab Mo x 1x20ul
8. p21 Waf1/Cip1 Mab Mo x 1x20ul
9. IgG(HRP) Pab Gt x Rb 1x100ul
10. IgG(HRP) Pab Gt x Mo 1x100ul

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.